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endogenous pcbp2 protein  (Proteintech)


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    Structured Review

    Proteintech endogenous pcbp2 protein
    Primers used for genes amplication.
    Endogenous Pcbp2 Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endogenous+pcbp2+protein/PCBP2+Fusion+Protein/pmc07355335-104-11-4
    Average 93 stars, based on 3 article reviews
    endogenous pcbp2 protein - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication"

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

    Journal: Veterinary Microbiology

    doi: 10.1016/j.vetmic.2020.108793

    Primers used for genes amplication.
    Figure Legend Snippet: Primers used for genes amplication.

    Techniques Used: Quantitative RT-PCR, Amplification


    Figure Legend Snippet: Identified proteins from immunoprecipitation reaction of PLP1 transfected cell lysates.

    Techniques Used: Immunoprecipitation, Transfection

    Identification of proteins that interact with PLP1 . HEK293 T cells were seeded in a six-well plate and cotransfected with pCMV-PLP1-HA, pECMV-3×FLAG-PCBP1, pECMV-3×FLAG-PCBP2 or empty vector. At 24 hpt, cells were lysed and immunoprecipitated with mouse anti-FLAG antibody. Immunoprecipitated samples were analyzed by western blotting.
    Figure Legend Snippet: Identification of proteins that interact with PLP1 . HEK293 T cells were seeded in a six-well plate and cotransfected with pCMV-PLP1-HA, pECMV-3×FLAG-PCBP1, pECMV-3×FLAG-PCBP2 or empty vector. At 24 hpt, cells were lysed and immunoprecipitated with mouse anti-FLAG antibody. Immunoprecipitated samples were analyzed by western blotting.

    Techniques Used: Plasmid Preparation, Immunoprecipitation, Western Blot

    Co-localization of PCBP2 with PLP1 . (A) Vero E6 cells were cotransfected with pCMV-PLP1-HA and pECMV-3×FLAG-PCBP2 or empty pCMV-HA vector. The cells were assessed using an indirect immunofluorescence assay with anti-HA and anti-FLAG antibodies. (B) Co-localization of PLP1 and endogenous PCBP2 in Vero E6 cells. The cells were transfected with pCMV-PLPL-HA or empty pCMV-HA vector and then subjected to the IFA with anti-HA and anti-PCBP2 antibodies.
    Figure Legend Snippet: Co-localization of PCBP2 with PLP1 . (A) Vero E6 cells were cotransfected with pCMV-PLP1-HA and pECMV-3×FLAG-PCBP2 or empty pCMV-HA vector. The cells were assessed using an indirect immunofluorescence assay with anti-HA and anti-FLAG antibodies. (B) Co-localization of PLP1 and endogenous PCBP2 in Vero E6 cells. The cells were transfected with pCMV-PLPL-HA or empty pCMV-HA vector and then subjected to the IFA with anti-HA and anti-PCBP2 antibodies.

    Techniques Used: Plasmid Preparation, Immunofluorescence, Transfection

    PLP1 up-regulates PCBP2 expression . Vero 6 cells were transfected with different concentrations of pCMV-PLP1-HA or empty pCMV-HA vector and western blotting was performed using anti-HA, anti-PCBP2 and anti-tubulin antibodies.
    Figure Legend Snippet: PLP1 up-regulates PCBP2 expression . Vero 6 cells were transfected with different concentrations of pCMV-PLP1-HA or empty pCMV-HA vector and western blotting was performed using anti-HA, anti-PCBP2 and anti-tubulin antibodies.

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Western Blot

    PCBP2 affects PEDV infection . IPEC-J2 cells were transfected with three different PCBP2-specific siRNAs. After 24 h, PCBP2 protein expression was assessed by western blotting with anti-PCBP2 and anti-tubulin antibodies. (B to C) IPEC-J2 cells were transfected with siRNA1, then 24 h later infected with PEDV for 24 h and 48 h. PCBP2 mRNA levels and PEDV RNA levels were determined by quantitative RT-PCR. (D) IPEC-J2 cells were transfected with pECMV-3×FLAG-PCBP2 or empty vector. At 24 hpt, the cells were infected with PEDV at a MOI of 0.1. PEDV viral loads were determined at 24, 48 and 60 hpi by quantitative RT-PCR.
    Figure Legend Snippet: PCBP2 affects PEDV infection . IPEC-J2 cells were transfected with three different PCBP2-specific siRNAs. After 24 h, PCBP2 protein expression was assessed by western blotting with anti-PCBP2 and anti-tubulin antibodies. (B to C) IPEC-J2 cells were transfected with siRNA1, then 24 h later infected with PEDV for 24 h and 48 h. PCBP2 mRNA levels and PEDV RNA levels were determined by quantitative RT-PCR. (D) IPEC-J2 cells were transfected with pECMV-3×FLAG-PCBP2 or empty vector. At 24 hpt, the cells were infected with PEDV at a MOI of 0.1. PEDV viral loads were determined at 24, 48 and 60 hpi by quantitative RT-PCR.

    Techniques Used: Infection, Transfection, Expressing, Western Blot, Quantitative RT-PCR, Plasmid Preparation

    Related Articles

    Quantitative RT-PCR:

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication
    Article Snippet: An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.. Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.. Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.

    Amplification:

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication
    Article Snippet: An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.. Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.. Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.

    Immunoprecipitation:

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication
    Article Snippet: An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.. Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.. Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.

    Transfection:

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication
    Article Snippet: An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.. Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.. Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.

    Plasmid Preparation:

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication
    Article Snippet: An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.. Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.. Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.

    Western Blot:

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication
    Article Snippet: An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.. Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.. Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.

    Immunofluorescence:

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication
    Article Snippet: An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.. Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.. Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.

    Expressing:

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication
    Article Snippet: An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.. Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.. Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.

    Infection:

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication
    Article Snippet: An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.An anti-FLAG mouse antibody (Sigma-Aldrich, St. Louis, MO) was used to detect FLAG-tagged proteins and an anti-tubulin antibody (Cell Signaling Technology) was used to detect tubulin.. Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.. Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.Horseradish peroxidase-conjugated anti-mouse IgG and goat anti-rabbit IgG antibodies were used as secondary antibodies.



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    endogenous pcbp2 protein  (Proteintech)
    93
    Proteintech endogenous pcbp2 protein
    Primers used for genes amplication.
    Endogenous Pcbp2 Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endogenous+pcbp2+protein/PCBP2+Fusion+Protein/pmc07355335-104-11-4
    Average 93 stars, based on 1 article reviews
    endogenous pcbp2 protein - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Primers used for genes amplication.

    Journal: Veterinary Microbiology

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

    doi: 10.1016/j.vetmic.2020.108793

    Figure Lengend Snippet: Primers used for genes amplication.

    Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

    Techniques: Quantitative RT-PCR, Amplification

    Journal: Veterinary Microbiology

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

    doi: 10.1016/j.vetmic.2020.108793

    Figure Lengend Snippet: Identified proteins from immunoprecipitation reaction of PLP1 transfected cell lysates.

    Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

    Techniques: Immunoprecipitation, Transfection

    Identification of proteins that interact with PLP1 . HEK293 T cells were seeded in a six-well plate and cotransfected with pCMV-PLP1-HA, pECMV-3×FLAG-PCBP1, pECMV-3×FLAG-PCBP2 or empty vector. At 24 hpt, cells were lysed and immunoprecipitated with mouse anti-FLAG antibody. Immunoprecipitated samples were analyzed by western blotting.

    Journal: Veterinary Microbiology

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

    doi: 10.1016/j.vetmic.2020.108793

    Figure Lengend Snippet: Identification of proteins that interact with PLP1 . HEK293 T cells were seeded in a six-well plate and cotransfected with pCMV-PLP1-HA, pECMV-3×FLAG-PCBP1, pECMV-3×FLAG-PCBP2 or empty vector. At 24 hpt, cells were lysed and immunoprecipitated with mouse anti-FLAG antibody. Immunoprecipitated samples were analyzed by western blotting.

    Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

    Techniques: Plasmid Preparation, Immunoprecipitation, Western Blot

    Co-localization of PCBP2 with PLP1 . (A) Vero E6 cells were cotransfected with pCMV-PLP1-HA and pECMV-3×FLAG-PCBP2 or empty pCMV-HA vector. The cells were assessed using an indirect immunofluorescence assay with anti-HA and anti-FLAG antibodies. (B) Co-localization of PLP1 and endogenous PCBP2 in Vero E6 cells. The cells were transfected with pCMV-PLPL-HA or empty pCMV-HA vector and then subjected to the IFA with anti-HA and anti-PCBP2 antibodies.

    Journal: Veterinary Microbiology

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

    doi: 10.1016/j.vetmic.2020.108793

    Figure Lengend Snippet: Co-localization of PCBP2 with PLP1 . (A) Vero E6 cells were cotransfected with pCMV-PLP1-HA and pECMV-3×FLAG-PCBP2 or empty pCMV-HA vector. The cells were assessed using an indirect immunofluorescence assay with anti-HA and anti-FLAG antibodies. (B) Co-localization of PLP1 and endogenous PCBP2 in Vero E6 cells. The cells were transfected with pCMV-PLPL-HA or empty pCMV-HA vector and then subjected to the IFA with anti-HA and anti-PCBP2 antibodies.

    Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

    Techniques: Plasmid Preparation, Immunofluorescence, Transfection

    PLP1 up-regulates PCBP2 expression . Vero 6 cells were transfected with different concentrations of pCMV-PLP1-HA or empty pCMV-HA vector and western blotting was performed using anti-HA, anti-PCBP2 and anti-tubulin antibodies.

    Journal: Veterinary Microbiology

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

    doi: 10.1016/j.vetmic.2020.108793

    Figure Lengend Snippet: PLP1 up-regulates PCBP2 expression . Vero 6 cells were transfected with different concentrations of pCMV-PLP1-HA or empty pCMV-HA vector and western blotting was performed using anti-HA, anti-PCBP2 and anti-tubulin antibodies.

    Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

    Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot

    PCBP2 affects PEDV infection . IPEC-J2 cells were transfected with three different PCBP2-specific siRNAs. After 24 h, PCBP2 protein expression was assessed by western blotting with anti-PCBP2 and anti-tubulin antibodies. (B to C) IPEC-J2 cells were transfected with siRNA1, then 24 h later infected with PEDV for 24 h and 48 h. PCBP2 mRNA levels and PEDV RNA levels were determined by quantitative RT-PCR. (D) IPEC-J2 cells were transfected with pECMV-3×FLAG-PCBP2 or empty vector. At 24 hpt, the cells were infected with PEDV at a MOI of 0.1. PEDV viral loads were determined at 24, 48 and 60 hpi by quantitative RT-PCR.

    Journal: Veterinary Microbiology

    Article Title: Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

    doi: 10.1016/j.vetmic.2020.108793

    Figure Lengend Snippet: PCBP2 affects PEDV infection . IPEC-J2 cells were transfected with three different PCBP2-specific siRNAs. After 24 h, PCBP2 protein expression was assessed by western blotting with anti-PCBP2 and anti-tubulin antibodies. (B to C) IPEC-J2 cells were transfected with siRNA1, then 24 h later infected with PEDV for 24 h and 48 h. PCBP2 mRNA levels and PEDV RNA levels were determined by quantitative RT-PCR. (D) IPEC-J2 cells were transfected with pECMV-3×FLAG-PCBP2 or empty vector. At 24 hpt, the cells were infected with PEDV at a MOI of 0.1. PEDV viral loads were determined at 24, 48 and 60 hpi by quantitative RT-PCR.

    Article Snippet: Rabbit polyclonal PCBP2 antibody (Proteintech, Chicago, IL) was used to detect endogenous PCBP2 protein.

    Techniques: Infection, Transfection, Expressing, Western Blot, Quantitative RT-PCR, Plasmid Preparation